Composite

Part:BBa_M11079

Designed by: Forrest Purser   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-18)


Copper binding paired with Polystyrene binding

Contains circularly permuted OmpX protein that enables E.coli to bind to a polystyrene surface. This sequence also contains the surface display protein OmpC, which has been modified to include a copper binding protein (CutA) on the extracellular loop of the membrane protein. This system is designed to be used for removing copper from solution by adding E. coli bound to a polystyrene surface and containing the copper binding mechanism to the solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1345
    Illegal SpeI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1345
    Illegal SpeI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1345
    Illegal BglII site found at 164
    Illegal BglII site found at 1053
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1345
    Illegal SpeI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1345
    Illegal SpeI site found at 1504
    Illegal AgeI site found at 1171
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None