Composite
Part:BBa_M11079
Designed by: Forrest Purser Group: UtahState BE5930 - S09 UtahState BE5930 - S10 (2009-04-18)
Copper binding paired with Polystyrene binding
Contains circularly permuted OmpX protein that enables E.coli to bind to a polystyrene surface. This sequence also contains the surface display protein OmpC, which has been modified to include a copper binding protein (CutA) on the extracellular loop of the membrane protein. This system is designed to be used for removing copper from solution by adding E. coli bound to a polystyrene surface and containing the copper binding mechanism to the solution.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1345
Illegal SpeI site found at 1504 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1345
Illegal SpeI site found at 1504 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1345
Illegal BglII site found at 164
Illegal BglII site found at 1053 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1345
Illegal SpeI site found at 1504 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1345
Illegal SpeI site found at 1504
Illegal AgeI site found at 1171 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |